Then please share with your network. You must be logged in to post a comment. This site uses Akismet to reduce spam. Learn how your comment data is processed. Facebook Twitter LinkedIn More. Written by Nicola Parry. Log in to Reply. Leave a Comment Cancel Reply You must be logged in to post a comment. Share via. Copy Link. Dilute mineral acid hydrolysis releases ferric ions from protein bound tissue deposits, which, in the presence of ferrocyanide ions, is precipitated as the highly coloured and highly water-insoluble complex, potassium ferric ferrocyanide, Prussian blue.
Ferrous ions do not produce a coloured reaction product, thus are excluded from the visualisation. Tissue deposits containing ferric ions are invariably haemosiderin. The original method of Perls applied the ferrocyanide and acid as separate reagents. Asbestos is the name given to a special form of silica which exists in the form of long, thin, crystalline fibres. The asbestos fibres with their protein are known as "asbestos bodies" and the characteristic birefringence is lost. Technical Points.
A known positive control section must be used to ensure correct differentiation has been achieved. Neutral buffered formalin gives good results. Other fixatives may be used, but acidic fixatives, dichromate fixatives, and acidic decalcification fluids should be avoided.
These reagents will cause progressive hydrolytic loss of ferric ions from tissues, and a negative result must be viewed with suspicion. Bancrofti, S Kim Suvarna. Fixation of Tissues. In: Theory and practice of histological techniques by John D.
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